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Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition
A FLAG tag consisting of DYKDDDDK is an epitope tag that is frequently and widely used to detect recombinant proteins of interest. In this study, we performed a CRISPR-based genetic screening to identify factors involved in the detection of a FLAG-tagged misfolded model protein at the cell surface....
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099120/ https://www.ncbi.nlm.nih.gov/pubmed/33951064 http://dx.doi.org/10.1371/journal.pone.0250805 |