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Targeted metagenomics using next generation sequencing in laboratory diagnosis of culture negative endophthalmitis

To study the feasibility of 16S rRNA metagenomics using next generation sequencing (NGS) along with broad range PCR assay for 762 bp region of 16S rRNA gene with Sanger's sequencing, in microbial diagnosis of culture negative endophthalmitis. Vitreous fluid from 16 culture negative and one cult...

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Detalles Bibliográficos
Autores principales: Mishra, Deepanshi, Satpathy, Gita, Chawla, Rohan, Paliwal, Daizy, Panda, Subrat Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099753/
https://www.ncbi.nlm.nih.gov/pubmed/33997374
http://dx.doi.org/10.1016/j.heliyon.2021.e06780