Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor

Förster resonance energy transfer (FRET) between fluorescent proteins has become a common platform for designing genetically encoded biosensors. For live cell imaging, the acceptor-to-donor intensity ratio is most commonly used to readout FRET efficiency, which largely depends on the proximity betwe...

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Detalles Bibliográficos
Autores principales: Laskaratou, Danai, Fernández, Guillermo Solís, Coucke, Quinten, Fron, Eduard, Rocha, Susana, Hofkens, Johan, Hendrix, Jelle, Mizuno, Hideaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099864/
https://www.ncbi.nlm.nih.gov/pubmed/33953187
http://dx.doi.org/10.1038/s41467-021-22816-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099864/
https://www.ncbi.nlm.nih.gov/pubmed/33953187
http://dx.doi.org/10.1038/s41467-021-22816-7

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