Transient siRNA-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis

We present a protocol for in vivo siRNA-mediated knockdown of a gene of interest in mouse liver using systemic delivery via intravenous injection. We describe a step-by-step protocol for delivery of siRNA particles, with tips on how to optimize dosage. We detail steps for feeding/starving cycles as...

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Detalles Bibliográficos
Autores principales: Wrobel, Lidia, Siddiqi, Farah H., Rubinsztein, David C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102171/
https://www.ncbi.nlm.nih.gov/pubmed/33997814
http://dx.doi.org/10.1016/j.xpro.2021.100500

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102171/
https://www.ncbi.nlm.nih.gov/pubmed/33997814
http://dx.doi.org/10.1016/j.xpro.2021.100500

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