Transient siRNA-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis

We present a protocol for in vivo siRNA-mediated knockdown of a gene of interest in mouse liver using systemic delivery via intravenous injection. We describe a step-by-step protocol for delivery of siRNA particles, with tips on how to optimize dosage. We detail steps for feeding/starving cycles as...

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Detalles Bibliográficos
Autores principales: Wrobel, Lidia, Siddiqi, Farah H., Rubinsztein, David C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102171/
https://www.ncbi.nlm.nih.gov/pubmed/33997814
http://dx.doi.org/10.1016/j.xpro.2021.100500
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author Wrobel, Lidia
Siddiqi, Farah H.
Rubinsztein, David C.
author_facet Wrobel, Lidia
Siddiqi, Farah H.
Rubinsztein, David C.
author_sort Wrobel, Lidia
collection PubMed
description We present a protocol for in vivo siRNA-mediated knockdown of a gene of interest in mouse liver using systemic delivery via intravenous injection. We describe a step-by-step protocol for delivery of siRNA particles, with tips on how to optimize dosage. We detail steps for feeding/starving cycles as well as for liver tissue isolation, followed by gene expression analysis, measured at the mRNA and protein levels. For complete information on the generation and use of this protocol, please refer to Wrobel et al. (2020).
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spelling pubmed-81021712021-05-14 Transient siRNA-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis Wrobel, Lidia Siddiqi, Farah H. Rubinsztein, David C. STAR Protoc Protocol We present a protocol for in vivo siRNA-mediated knockdown of a gene of interest in mouse liver using systemic delivery via intravenous injection. We describe a step-by-step protocol for delivery of siRNA particles, with tips on how to optimize dosage. We detail steps for feeding/starving cycles as well as for liver tissue isolation, followed by gene expression analysis, measured at the mRNA and protein levels. For complete information on the generation and use of this protocol, please refer to Wrobel et al. (2020). Elsevier 2021-04-27 /pmc/articles/PMC8102171/ /pubmed/33997814 http://dx.doi.org/10.1016/j.xpro.2021.100500 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Wrobel, Lidia
Siddiqi, Farah H.
Rubinsztein, David C.
Transient siRNA-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis
title Transient siRNA-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis
title_full Transient siRNA-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis
title_fullStr Transient siRNA-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis
title_full_unstemmed Transient siRNA-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis
title_short Transient siRNA-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis
title_sort transient sirna-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102171/
https://www.ncbi.nlm.nih.gov/pubmed/33997814
http://dx.doi.org/10.1016/j.xpro.2021.100500
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