Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells

The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry, suitable to specifically map the content of autophagosomes...

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Detalles Bibliográficos
Autores principales: Zellner, Susanne, Nalbach, Karsten, Behrends, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102175/
https://www.ncbi.nlm.nih.gov/pubmed/33997820
http://dx.doi.org/10.1016/j.xpro.2021.100506

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102175/
https://www.ncbi.nlm.nih.gov/pubmed/33997820
http://dx.doi.org/10.1016/j.xpro.2021.100506

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