Cargando…
Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells
The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry, suitable to specifically map the content of autophagosomes...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102175/ https://www.ncbi.nlm.nih.gov/pubmed/33997820 http://dx.doi.org/10.1016/j.xpro.2021.100506 |