Water pre-filtration methods to improve environmental DNA detection by real-time PCR and metabarcoding

Environmental DNA (eDNA) analysis is a novel approach for biomonitoring and has been mostly used in clear water. It is difficult to detect eDNA in turbid water as filter clogging occurs, and environmental samples contain various substances that inhibit the polymerase chain reaction (PCR) and affect...

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Detalles Bibliográficos
Autores principales: Takasaki, Kazuto, Aihara, Hiroki, Imanaka, Takanobu, Matsudaira, Takahiro, Tsukahara, Keita, Usui, Atsuko, Osaki, Sora, Doi, Hideyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104373/
https://www.ncbi.nlm.nih.gov/pubmed/33961651
http://dx.doi.org/10.1371/journal.pone.0250162

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104373/
https://www.ncbi.nlm.nih.gov/pubmed/33961651
http://dx.doi.org/10.1371/journal.pone.0250162

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