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Water pre-filtration methods to improve environmental DNA detection by real-time PCR and metabarcoding
Environmental DNA (eDNA) analysis is a novel approach for biomonitoring and has been mostly used in clear water. It is difficult to detect eDNA in turbid water as filter clogging occurs, and environmental samples contain various substances that inhibit the polymerase chain reaction (PCR) and affect...
Autores principales: | Takasaki, Kazuto, Aihara, Hiroki, Imanaka, Takanobu, Matsudaira, Takahiro, Tsukahara, Keita, Usui, Atsuko, Osaki, Sora, Doi, Hideyuki |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104373/ https://www.ncbi.nlm.nih.gov/pubmed/33961651 http://dx.doi.org/10.1371/journal.pone.0250162 |
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