A robust method for particulate detection of a genetic tag for 3D electron microscopy

Genetic tags allow rapid localization of tagged proteins in cells and tissues. APEX, an ascorbate peroxidase, has proven to be one of the most versatile and robust genetic tags for ultrastructural localization by electron microscopy (EM). Here, we describe a simple method, APEX-Gold, which converts...

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Detalles Bibliográficos
Autores principales: Rae, James, Ferguson, Charles, Ariotti, Nicholas, Webb, Richard I, Cheng, Han-Hao, Mead, James L, Riches, James D, Hunter, Dominic JB, Martel, Nick, Baltos, Joanne, Christopoulos, Arthur, Bryce, Nicole S, Cagigas, Maria Lastra, Fonseka, Sachini, Sayre, Marcel E, Hardeman, Edna C, Gunning, Peter W, Gambin, Yann, Hall, Thomas E, Parton, Robert G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104959/
https://www.ncbi.nlm.nih.gov/pubmed/33904409
http://dx.doi.org/10.7554/eLife.64630

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104959/
https://www.ncbi.nlm.nih.gov/pubmed/33904409
http://dx.doi.org/10.7554/eLife.64630

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