Protocol for automated production of human stem cell derived liver spheres

This protocol describes how to produce human liver spheres from pluripotent stem cell-derived hepatic progenitors, endothelial cells, and hepatic stellate cells. Liver spheres form by self-assembly in microwells, generating up to 73 spheres per well of a 96-well plate. This process was automated usi...

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Detalles Bibliográficos
Autores principales: Meseguer-Ripolles, Jose, Kasarinaite, Alvile, Lucendo-Villarin, Baltasar, Hay, David C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105683/
https://www.ncbi.nlm.nih.gov/pubmed/33997816
http://dx.doi.org/10.1016/j.xpro.2021.100502

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105683/
https://www.ncbi.nlm.nih.gov/pubmed/33997816
http://dx.doi.org/10.1016/j.xpro.2021.100502

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