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Sequence-specific inhibition of reverse transcription by recombinant CRISPR/dCas13a ribonucleoprotein complexes in vitro

The clustered regularly interspaced short palindromic repeats (CRISPR) system is widely used for genome editing because of its ability to cleave specific DNA sequences. Recently, RNA-specific CRISPR systems have been reported. CRISPR systems, consisting of a guide RNA (gRNA) and a nuclease-dead form...

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Detalles Bibliográficos
Autores principales: Fujita, Toshitsugu, Nagata, Shoko, Yuno, Miyuki, Fujii, Hodaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106441/
https://www.ncbi.nlm.nih.gov/pubmed/33981854
http://dx.doi.org/10.1093/biomethods/bpab009