A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells
PURPOSE: McCarey-Kaufman’s (MK) medium and Optisol-GS medium are the most commonly employed media for human donor corneal preservation. In this study, we evaluated the preservation efficacy of discarded human donor corneas using a Thermo-reversible gelation polymer (TGP) added to these two media. ME...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Netherlands
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113287/ https://www.ncbi.nlm.nih.gov/pubmed/33768381 http://dx.doi.org/10.1007/s10529-021-03116-y |
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author | Namitha, Bhuvaneshwari Chitra, Munusamy Rajendran Bhavya, Mathevan Parikumar, Periasamy Katoh, Shojiro Yoshioka, Hiroshi Iwasaki, Masaru Senthilkumar, Rajappa Rajmohan, Mathaiyan Karthick, Ramalingam Preethy, Senthilkumar Abraham, Samuel J. K. |
author_facet | Namitha, Bhuvaneshwari Chitra, Munusamy Rajendran Bhavya, Mathevan Parikumar, Periasamy Katoh, Shojiro Yoshioka, Hiroshi Iwasaki, Masaru Senthilkumar, Rajappa Rajmohan, Mathaiyan Karthick, Ramalingam Preethy, Senthilkumar Abraham, Samuel J. K. |
author_sort | Namitha, Bhuvaneshwari |
collection | PubMed |
description | PURPOSE: McCarey-Kaufman’s (MK) medium and Optisol-GS medium are the most commonly employed media for human donor corneal preservation. In this study, we evaluated the preservation efficacy of discarded human donor corneas using a Thermo-reversible gelation polymer (TGP) added to these two media. METHODS: Thirteen human corneal buttons collected from deceased donors, which were otherwise discarded due to low endothelial cell density (ECD) were used. They were stored in four groups: MK medium, MK medium with TGP, Optisol-GS and Optisol-GS with TGP at 4 °C for 96 h. Slit lamp examination and specular microscopy were performed. Corneal limbal tissues from these corneas were then cultured using explant methodology one with and the other without TGP scaffold, for 21 days. RESULTS: MK + TGP and Optisol-GS + TGP preserved corneas better than without TGP, which was observed by maintenance of ECD which was significantly higher in Optisol-GS + TGP than MK + TGP (p-value = 0.000478) and corneal thickness remaining the same for 96 h. Viable corneal epithelial cells could be grown from the corneas stored only in MK + TGP and Optisol-GS + TGP. During culture, the TGP scaffold helped maintain the native epithelial phenotype and progenitor/stem cell growth was confirmed by RT-PCR characterization. CONCLUSION: TGP reconstituted with MK and Optisol—GS media yields better preservation of human corneal buttons in terms of relatively higher ECD maintenance and better in vitro culture outcome of corneal limbal tissue. This method has the potential to become a standard donor corneal transportation-preservation methodology and it can also be extended to other tissue or organ transportation upon further validation. |
format | Online Article Text |
id | pubmed-8113287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-81132872021-05-13 A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells Namitha, Bhuvaneshwari Chitra, Munusamy Rajendran Bhavya, Mathevan Parikumar, Periasamy Katoh, Shojiro Yoshioka, Hiroshi Iwasaki, Masaru Senthilkumar, Rajappa Rajmohan, Mathaiyan Karthick, Ramalingam Preethy, Senthilkumar Abraham, Samuel J. K. Biotechnol Lett Original Research Paper PURPOSE: McCarey-Kaufman’s (MK) medium and Optisol-GS medium are the most commonly employed media for human donor corneal preservation. In this study, we evaluated the preservation efficacy of discarded human donor corneas using a Thermo-reversible gelation polymer (TGP) added to these two media. METHODS: Thirteen human corneal buttons collected from deceased donors, which were otherwise discarded due to low endothelial cell density (ECD) were used. They were stored in four groups: MK medium, MK medium with TGP, Optisol-GS and Optisol-GS with TGP at 4 °C for 96 h. Slit lamp examination and specular microscopy were performed. Corneal limbal tissues from these corneas were then cultured using explant methodology one with and the other without TGP scaffold, for 21 days. RESULTS: MK + TGP and Optisol-GS + TGP preserved corneas better than without TGP, which was observed by maintenance of ECD which was significantly higher in Optisol-GS + TGP than MK + TGP (p-value = 0.000478) and corneal thickness remaining the same for 96 h. Viable corneal epithelial cells could be grown from the corneas stored only in MK + TGP and Optisol-GS + TGP. During culture, the TGP scaffold helped maintain the native epithelial phenotype and progenitor/stem cell growth was confirmed by RT-PCR characterization. CONCLUSION: TGP reconstituted with MK and Optisol—GS media yields better preservation of human corneal buttons in terms of relatively higher ECD maintenance and better in vitro culture outcome of corneal limbal tissue. This method has the potential to become a standard donor corneal transportation-preservation methodology and it can also be extended to other tissue or organ transportation upon further validation. Springer Netherlands 2021-03-25 2021 /pmc/articles/PMC8113287/ /pubmed/33768381 http://dx.doi.org/10.1007/s10529-021-03116-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Research Paper Namitha, Bhuvaneshwari Chitra, Munusamy Rajendran Bhavya, Mathevan Parikumar, Periasamy Katoh, Shojiro Yoshioka, Hiroshi Iwasaki, Masaru Senthilkumar, Rajappa Rajmohan, Mathaiyan Karthick, Ramalingam Preethy, Senthilkumar Abraham, Samuel J. K. A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells |
title | A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells |
title_full | A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells |
title_fullStr | A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells |
title_full_unstemmed | A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells |
title_short | A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells |
title_sort | novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (tgp), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells |
topic | Original Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113287/ https://www.ncbi.nlm.nih.gov/pubmed/33768381 http://dx.doi.org/10.1007/s10529-021-03116-y |
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