A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells

PURPOSE: McCarey-Kaufman’s (MK) medium and Optisol-GS medium are the most commonly employed media for human donor corneal preservation. In this study, we evaluated the preservation efficacy of discarded human donor corneas using a Thermo-reversible gelation polymer (TGP) added to these two media. ME...

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Autores principales: Namitha, Bhuvaneshwari, Chitra, Munusamy Rajendran, Bhavya, Mathevan, Parikumar, Periasamy, Katoh, Shojiro, Yoshioka, Hiroshi, Iwasaki, Masaru, Senthilkumar, Rajappa, Rajmohan, Mathaiyan, Karthick, Ramalingam, Preethy, Senthilkumar, Abraham, Samuel J. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113287/
https://www.ncbi.nlm.nih.gov/pubmed/33768381
http://dx.doi.org/10.1007/s10529-021-03116-y
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author Namitha, Bhuvaneshwari
Chitra, Munusamy Rajendran
Bhavya, Mathevan
Parikumar, Periasamy
Katoh, Shojiro
Yoshioka, Hiroshi
Iwasaki, Masaru
Senthilkumar, Rajappa
Rajmohan, Mathaiyan
Karthick, Ramalingam
Preethy, Senthilkumar
Abraham, Samuel J. K.
author_facet Namitha, Bhuvaneshwari
Chitra, Munusamy Rajendran
Bhavya, Mathevan
Parikumar, Periasamy
Katoh, Shojiro
Yoshioka, Hiroshi
Iwasaki, Masaru
Senthilkumar, Rajappa
Rajmohan, Mathaiyan
Karthick, Ramalingam
Preethy, Senthilkumar
Abraham, Samuel J. K.
author_sort Namitha, Bhuvaneshwari
collection PubMed
description PURPOSE: McCarey-Kaufman’s (MK) medium and Optisol-GS medium are the most commonly employed media for human donor corneal preservation. In this study, we evaluated the preservation efficacy of discarded human donor corneas using a Thermo-reversible gelation polymer (TGP) added to these two media. METHODS: Thirteen human corneal buttons collected from deceased donors, which were otherwise discarded due to low endothelial cell density (ECD) were used. They were stored in four groups: MK medium, MK medium with TGP, Optisol-GS and Optisol-GS with TGP at 4 °C for 96 h. Slit lamp examination and specular microscopy were performed. Corneal limbal tissues from these corneas were then cultured using explant methodology one with and the other without TGP scaffold, for 21 days. RESULTS: MK + TGP and Optisol-GS + TGP preserved corneas better than without TGP, which was observed by maintenance of ECD which was significantly higher in Optisol-GS + TGP than MK + TGP (p-value = 0.000478) and corneal thickness remaining the same for 96 h. Viable corneal epithelial cells could be grown from the corneas stored only in MK + TGP and Optisol-GS + TGP. During culture, the TGP scaffold helped maintain the native epithelial phenotype and progenitor/stem cell growth was confirmed by RT-PCR characterization. CONCLUSION: TGP reconstituted with MK and Optisol—GS media yields better preservation of human corneal buttons in terms of relatively higher ECD maintenance and better in vitro culture outcome of corneal limbal tissue. This method has the potential to become a standard donor corneal transportation-preservation methodology and it can also be extended to other tissue or organ transportation upon further validation.
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spelling pubmed-81132872021-05-13 A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells Namitha, Bhuvaneshwari Chitra, Munusamy Rajendran Bhavya, Mathevan Parikumar, Periasamy Katoh, Shojiro Yoshioka, Hiroshi Iwasaki, Masaru Senthilkumar, Rajappa Rajmohan, Mathaiyan Karthick, Ramalingam Preethy, Senthilkumar Abraham, Samuel J. K. Biotechnol Lett Original Research Paper PURPOSE: McCarey-Kaufman’s (MK) medium and Optisol-GS medium are the most commonly employed media for human donor corneal preservation. In this study, we evaluated the preservation efficacy of discarded human donor corneas using a Thermo-reversible gelation polymer (TGP) added to these two media. METHODS: Thirteen human corneal buttons collected from deceased donors, which were otherwise discarded due to low endothelial cell density (ECD) were used. They were stored in four groups: MK medium, MK medium with TGP, Optisol-GS and Optisol-GS with TGP at 4 °C for 96 h. Slit lamp examination and specular microscopy were performed. Corneal limbal tissues from these corneas were then cultured using explant methodology one with and the other without TGP scaffold, for 21 days. RESULTS: MK + TGP and Optisol-GS + TGP preserved corneas better than without TGP, which was observed by maintenance of ECD which was significantly higher in Optisol-GS + TGP than MK + TGP (p-value = 0.000478) and corneal thickness remaining the same for 96 h. Viable corneal epithelial cells could be grown from the corneas stored only in MK + TGP and Optisol-GS + TGP. During culture, the TGP scaffold helped maintain the native epithelial phenotype and progenitor/stem cell growth was confirmed by RT-PCR characterization. CONCLUSION: TGP reconstituted with MK and Optisol—GS media yields better preservation of human corneal buttons in terms of relatively higher ECD maintenance and better in vitro culture outcome of corneal limbal tissue. This method has the potential to become a standard donor corneal transportation-preservation methodology and it can also be extended to other tissue or organ transportation upon further validation. Springer Netherlands 2021-03-25 2021 /pmc/articles/PMC8113287/ /pubmed/33768381 http://dx.doi.org/10.1007/s10529-021-03116-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Research Paper
Namitha, Bhuvaneshwari
Chitra, Munusamy Rajendran
Bhavya, Mathevan
Parikumar, Periasamy
Katoh, Shojiro
Yoshioka, Hiroshi
Iwasaki, Masaru
Senthilkumar, Rajappa
Rajmohan, Mathaiyan
Karthick, Ramalingam
Preethy, Senthilkumar
Abraham, Samuel J. K.
A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells
title A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells
title_full A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells
title_fullStr A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells
title_full_unstemmed A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells
title_short A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells
title_sort novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (tgp), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113287/
https://www.ncbi.nlm.nih.gov/pubmed/33768381
http://dx.doi.org/10.1007/s10529-021-03116-y
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