CRISPR screen to determine the in vivo fitness of Toxoplasma genes

The virulence of eukaryotic parasites like Toxoplasma gondii depends on their capacity to escape from the host immune response and disseminate throughout the host organism. However, Toxoplasma gene products essential for its in vivo pathogenesis remain uncharacterized. Here, we present the complete...

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Autores principales: Sangaré, Lamba Omar, Wang, Yifan, Arranz-Solís, David, Saeij, Jeroen P.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113973/
https://www.ncbi.nlm.nih.gov/pubmed/34013213
http://dx.doi.org/10.1016/j.xpro.2021.100520
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author Sangaré, Lamba Omar
Wang, Yifan
Arranz-Solís, David
Saeij, Jeroen P.J.
author_facet Sangaré, Lamba Omar
Wang, Yifan
Arranz-Solís, David
Saeij, Jeroen P.J.
author_sort Sangaré, Lamba Omar
collection PubMed
description The virulence of eukaryotic parasites like Toxoplasma gondii depends on their capacity to escape from the host immune response and disseminate throughout the host organism. However, Toxoplasma gene products essential for its in vivo pathogenesis remain uncharacterized. Here, we present the complete workflow of a CRISPR-Cas9 in vivo loss-of-function screen to identify Toxoplasma fitness-conferring genes. This protocol can be used to uncover gene products that play a role in Toxoplasma immune evasion, nutrient acquisition, dissemination, and tissue colonization. For complete details on the use and execution of this protocol, please refer to Sangaré et al. (2019).
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spelling pubmed-81139732021-05-18 CRISPR screen to determine the in vivo fitness of Toxoplasma genes Sangaré, Lamba Omar Wang, Yifan Arranz-Solís, David Saeij, Jeroen P.J. STAR Protoc Protocol The virulence of eukaryotic parasites like Toxoplasma gondii depends on their capacity to escape from the host immune response and disseminate throughout the host organism. However, Toxoplasma gene products essential for its in vivo pathogenesis remain uncharacterized. Here, we present the complete workflow of a CRISPR-Cas9 in vivo loss-of-function screen to identify Toxoplasma fitness-conferring genes. This protocol can be used to uncover gene products that play a role in Toxoplasma immune evasion, nutrient acquisition, dissemination, and tissue colonization. For complete details on the use and execution of this protocol, please refer to Sangaré et al. (2019). Elsevier 2021-05-03 /pmc/articles/PMC8113973/ /pubmed/34013213 http://dx.doi.org/10.1016/j.xpro.2021.100520 Text en © 2021. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sangaré, Lamba Omar
Wang, Yifan
Arranz-Solís, David
Saeij, Jeroen P.J.
CRISPR screen to determine the in vivo fitness of Toxoplasma genes
title CRISPR screen to determine the in vivo fitness of Toxoplasma genes
title_full CRISPR screen to determine the in vivo fitness of Toxoplasma genes
title_fullStr CRISPR screen to determine the in vivo fitness of Toxoplasma genes
title_full_unstemmed CRISPR screen to determine the in vivo fitness of Toxoplasma genes
title_short CRISPR screen to determine the in vivo fitness of Toxoplasma genes
title_sort crispr screen to determine the in vivo fitness of toxoplasma genes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113973/
https://www.ncbi.nlm.nih.gov/pubmed/34013213
http://dx.doi.org/10.1016/j.xpro.2021.100520
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AT saeijjeroenpj crisprscreentodeterminetheinvivofitnessoftoxoplasmagenes

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