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Trans Fatty Acid Intake Induces Intestinal Inflammation and Impaired Glucose Tolerance

BACKGROUND AND AIMS: Many nutritional and epidemiological studies have shown that high consumption of trans fatty acids can cause several adverse effects on human health, including cardiovascular disease, diabetes, and cancer. In the present study, we investigated the effect of trans fatty acids on...

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Autores principales: Okamura, Takuro, Hashimoto, Yoshitaka, Majima, Saori, Senmaru, Takafumi, Ushigome, Emi, Nakanishi, Naoko, Asano, Mai, Yamazaki, Masahiro, Takakuwa, Hiroshi, Hamaguchi, Masahide, Fukui, Michiaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117213/
https://www.ncbi.nlm.nih.gov/pubmed/33995404
http://dx.doi.org/10.3389/fimmu.2021.669672
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author Okamura, Takuro
Hashimoto, Yoshitaka
Majima, Saori
Senmaru, Takafumi
Ushigome, Emi
Nakanishi, Naoko
Asano, Mai
Yamazaki, Masahiro
Takakuwa, Hiroshi
Hamaguchi, Masahide
Fukui, Michiaki
author_facet Okamura, Takuro
Hashimoto, Yoshitaka
Majima, Saori
Senmaru, Takafumi
Ushigome, Emi
Nakanishi, Naoko
Asano, Mai
Yamazaki, Masahiro
Takakuwa, Hiroshi
Hamaguchi, Masahide
Fukui, Michiaki
author_sort Okamura, Takuro
collection PubMed
description BACKGROUND AND AIMS: Many nutritional and epidemiological studies have shown that high consumption of trans fatty acids can cause several adverse effects on human health, including cardiovascular disease, diabetes, and cancer. In the present study, we investigated the effect of trans fatty acids on innate immunity in the gut by observing mice fed with a diet high in trans fatty acids, which have been reported to cause dysbiosis. METHODS: We used C57BL6/J mice and fed them with normal diet (ND) or high-fat, high-sucrose diet (HFHSD) or high-trans fatty acid, high-sucrose diet (HTHSD) for 12 weeks. 16S rRNA gene sequencing was performed on the mice stool samples, in addition to flow cytometry, real-time PCR, and lipidomics analysis of the mice serum and liver samples. RAW264.7 cells were used for the in vitro studies. RESULTS: Mice fed with HTHSD displayed significantly higher blood glucose levels and advanced fatty liver and intestinal inflammation, as compared to mice fed with HFHSD. Furthermore, compared to mice fed with HFHSD, mice fed with HTHSD displayed a significant elevation in the expression of CD36 in the small intestine, along with a reduction in the expression of IL-22. Furthermore, there was a significant increase in the populations of ILC1s and T-bet-positive ILC3s in the lamina propria in mice fed with HTHSD. Finally, the relative abundance of the family Desulfovibrionaceae, which belongs to the phylum Proteobacteria, was significantly higher in mice fed with HFHSD or HTHSD, than in mice fed with ND; between the HFHSD and HTHSD groups, the abundance was slightly higher in the HTHSD group. CONCLUSIONS: This study revealed that compared to saturated fatty acid intake, trans fatty acid intake significantly exacerbated metabolic diseases such as diabetes and fatty liver.
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spelling pubmed-81172132021-05-14 Trans Fatty Acid Intake Induces Intestinal Inflammation and Impaired Glucose Tolerance Okamura, Takuro Hashimoto, Yoshitaka Majima, Saori Senmaru, Takafumi Ushigome, Emi Nakanishi, Naoko Asano, Mai Yamazaki, Masahiro Takakuwa, Hiroshi Hamaguchi, Masahide Fukui, Michiaki Front Immunol Immunology BACKGROUND AND AIMS: Many nutritional and epidemiological studies have shown that high consumption of trans fatty acids can cause several adverse effects on human health, including cardiovascular disease, diabetes, and cancer. In the present study, we investigated the effect of trans fatty acids on innate immunity in the gut by observing mice fed with a diet high in trans fatty acids, which have been reported to cause dysbiosis. METHODS: We used C57BL6/J mice and fed them with normal diet (ND) or high-fat, high-sucrose diet (HFHSD) or high-trans fatty acid, high-sucrose diet (HTHSD) for 12 weeks. 16S rRNA gene sequencing was performed on the mice stool samples, in addition to flow cytometry, real-time PCR, and lipidomics analysis of the mice serum and liver samples. RAW264.7 cells were used for the in vitro studies. RESULTS: Mice fed with HTHSD displayed significantly higher blood glucose levels and advanced fatty liver and intestinal inflammation, as compared to mice fed with HFHSD. Furthermore, compared to mice fed with HFHSD, mice fed with HTHSD displayed a significant elevation in the expression of CD36 in the small intestine, along with a reduction in the expression of IL-22. Furthermore, there was a significant increase in the populations of ILC1s and T-bet-positive ILC3s in the lamina propria in mice fed with HTHSD. Finally, the relative abundance of the family Desulfovibrionaceae, which belongs to the phylum Proteobacteria, was significantly higher in mice fed with HFHSD or HTHSD, than in mice fed with ND; between the HFHSD and HTHSD groups, the abundance was slightly higher in the HTHSD group. CONCLUSIONS: This study revealed that compared to saturated fatty acid intake, trans fatty acid intake significantly exacerbated metabolic diseases such as diabetes and fatty liver. Frontiers Media S.A. 2021-04-29 /pmc/articles/PMC8117213/ /pubmed/33995404 http://dx.doi.org/10.3389/fimmu.2021.669672 Text en Copyright © 2021 Okamura, Hashimoto, Majima, Senmaru, Ushigome, Nakanishi, Asano, Yamazaki, Takakuwa, Hamaguchi and Fukui https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Okamura, Takuro
Hashimoto, Yoshitaka
Majima, Saori
Senmaru, Takafumi
Ushigome, Emi
Nakanishi, Naoko
Asano, Mai
Yamazaki, Masahiro
Takakuwa, Hiroshi
Hamaguchi, Masahide
Fukui, Michiaki
Trans Fatty Acid Intake Induces Intestinal Inflammation and Impaired Glucose Tolerance
title Trans Fatty Acid Intake Induces Intestinal Inflammation and Impaired Glucose Tolerance
title_full Trans Fatty Acid Intake Induces Intestinal Inflammation and Impaired Glucose Tolerance
title_fullStr Trans Fatty Acid Intake Induces Intestinal Inflammation and Impaired Glucose Tolerance
title_full_unstemmed Trans Fatty Acid Intake Induces Intestinal Inflammation and Impaired Glucose Tolerance
title_short Trans Fatty Acid Intake Induces Intestinal Inflammation and Impaired Glucose Tolerance
title_sort trans fatty acid intake induces intestinal inflammation and impaired glucose tolerance
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117213/
https://www.ncbi.nlm.nih.gov/pubmed/33995404
http://dx.doi.org/10.3389/fimmu.2021.669672
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