3-D Human Renal Tubular Organoids Generated from Urine-Derived Stem Cells for Nephrotoxicity Screening

The development of human cell-based systems to replace the use of rodents or the two-dimensional culture of cells for studying nephrotoxicity is urgently needed. Human urine-derived stem cells were differentiated into renal tubular epithelial cells in three-dimensional (3-D) culture after being indu...

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Detalles Bibliográficos
Autores principales: Guo, Haibin, Deng, Nan, Dou, Lei, Ding, Huifen, Criswell, Tracy, Atala, Anthony, Furdui, Cristina M., Zhang, Yuanyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118570/
https://www.ncbi.nlm.nih.gov/pubmed/33320634
http://dx.doi.org/10.1021/acsbiomaterials.0c01468
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author Guo, Haibin
Deng, Nan
Dou, Lei
Ding, Huifen
Criswell, Tracy
Atala, Anthony
Furdui, Cristina M.
Zhang, Yuanyuan
author_facet Guo, Haibin
Deng, Nan
Dou, Lei
Ding, Huifen
Criswell, Tracy
Atala, Anthony
Furdui, Cristina M.
Zhang, Yuanyuan
author_sort Guo, Haibin
collection PubMed
description The development of human cell-based systems to replace the use of rodents or the two-dimensional culture of cells for studying nephrotoxicity is urgently needed. Human urine-derived stem cells were differentiated into renal tubular epithelial cells in three-dimensional (3-D) culture after being induced by a kidney extracellular matrix. Levels of CYP2E1 and KIM-1 in 3-D organoids were significantly increased in response to acetone and cisplatin. This 3-D culture system provides an alternative tool for nephrotoxicity screening and research.
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spelling pubmed-81185702021-05-13 3-D Human Renal Tubular Organoids Generated from Urine-Derived Stem Cells for Nephrotoxicity Screening Guo, Haibin Deng, Nan Dou, Lei Ding, Huifen Criswell, Tracy Atala, Anthony Furdui, Cristina M. Zhang, Yuanyuan ACS Biomater Sci Eng Article The development of human cell-based systems to replace the use of rodents or the two-dimensional culture of cells for studying nephrotoxicity is urgently needed. Human urine-derived stem cells were differentiated into renal tubular epithelial cells in three-dimensional (3-D) culture after being induced by a kidney extracellular matrix. Levels of CYP2E1 and KIM-1 in 3-D organoids were significantly increased in response to acetone and cisplatin. This 3-D culture system provides an alternative tool for nephrotoxicity screening and research. 2020-11-13 2020-12-14 /pmc/articles/PMC8118570/ /pubmed/33320634 http://dx.doi.org/10.1021/acsbiomaterials.0c01468 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (https://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Article
Guo, Haibin
Deng, Nan
Dou, Lei
Ding, Huifen
Criswell, Tracy
Atala, Anthony
Furdui, Cristina M.
Zhang, Yuanyuan
3-D Human Renal Tubular Organoids Generated from Urine-Derived Stem Cells for Nephrotoxicity Screening
title 3-D Human Renal Tubular Organoids Generated from Urine-Derived Stem Cells for Nephrotoxicity Screening
title_full 3-D Human Renal Tubular Organoids Generated from Urine-Derived Stem Cells for Nephrotoxicity Screening
title_fullStr 3-D Human Renal Tubular Organoids Generated from Urine-Derived Stem Cells for Nephrotoxicity Screening
title_full_unstemmed 3-D Human Renal Tubular Organoids Generated from Urine-Derived Stem Cells for Nephrotoxicity Screening
title_short 3-D Human Renal Tubular Organoids Generated from Urine-Derived Stem Cells for Nephrotoxicity Screening
title_sort 3-d human renal tubular organoids generated from urine-derived stem cells for nephrotoxicity screening
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118570/
https://www.ncbi.nlm.nih.gov/pubmed/33320634
http://dx.doi.org/10.1021/acsbiomaterials.0c01468
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