The effects of beta-cell mass and function, intercellular coupling, and islet synchrony on [Formula: see text] dynamics

Type 2 diabetes (T2D) is a challenging metabolic disorder characterized by a substantial loss of [Formula: see text] -cell mass and alteration of [Formula: see text] -cell function in the islets of Langerhans, disrupting insulin secretion and glucose homeostasis. The mechanisms for deficiency in [Formula: see text] -cell mass and function during the hyperglycemia development and T2D pathogenesis are complex. To study the relative contribution of [Formula: see text] -cell mass to [Formula: see text] -cell function in T2D, we make use of a comprehensive electrophysiological model of human [Formula: see text] -cell clusters. We find that defect in [Formula: see text] -cell mass causes a functional decline in single [Formula: see text] -cell, impairment in intra-islet synchrony, and changes in the form of oscillatory patterns of membrane potential and intracellular [Formula: see text] concentration, which can lead to changes in insulin secretion dynamics and in insulin levels. The model demonstrates a good correspondence between suppression of synchronizing electrical activity and published experimental measurements. We then compare the role of gap junction-mediated electrical coupling with both [Formula: see text] -cell synchronization and metabolic coupling in the behavior of [Formula: see text] concentration dynamics within human islets. Our results indicate that inter-[Formula: see text] -cellular electrical coupling depicts a more important factor in shaping the physiological regulation of islet function and in human T2D. We further predict that varying the whole-cell conductance of delayed rectifier [Formula: see text] channels modifies oscillatory activity patterns of [Formula: see text] -cell population lacking intercellular coupling, which significantly affect [Formula: see text] concentration and insulin secretion.