A screening system for identifying interacting proteins using biomolecular fluorescence complementation and transposon gene trap

We have established a new screening system for identifying interacting proteins by combining biomolecular fluorescence complementation (BiFC) and a transposon gene trap system. This system requires creation of a bait strain that stably expresses a fusion product of part of the fluorescent monomeric...

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Detalles Bibliográficos
Autores principales: Miyakura, Honami, Fukuda, Mei, Enomoto, Hiroya, Ishikawa, Kosuke, Watanabe, Shinya, Semba, Kentaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121353/
https://www.ncbi.nlm.nih.gov/pubmed/33989302
http://dx.doi.org/10.1371/journal.pone.0251240

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121353/
https://www.ncbi.nlm.nih.gov/pubmed/33989302
http://dx.doi.org/10.1371/journal.pone.0251240

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