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A screening system for identifying interacting proteins using biomolecular fluorescence complementation and transposon gene trap
We have established a new screening system for identifying interacting proteins by combining biomolecular fluorescence complementation (BiFC) and a transposon gene trap system. This system requires creation of a bait strain that stably expresses a fusion product of part of the fluorescent monomeric...
Autores principales: | Miyakura, Honami, Fukuda, Mei, Enomoto, Hiroya, Ishikawa, Kosuke, Watanabe, Shinya, Semba, Kentaro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121353/ https://www.ncbi.nlm.nih.gov/pubmed/33989302 http://dx.doi.org/10.1371/journal.pone.0251240 |
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