Allosteric communication in DNA polymerase clamp loaders relies on a critical hydrogen-bonded junction

Clamp loaders are AAA+ ATPases that load sliding clamps onto DNA. We mapped the mutational sensitivity of the T4 bacteriophage sliding clamp and clamp loader by deep mutagenesis, and found that residues not involved in catalysis or binding display remarkable tolerance to mutation. An exception is a...

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Detalles Bibliográficos
Autores principales: Subramanian, Subu, Gorday, Kent, Marcus, Kendra, Orellana, Matthew R, Ren, Peter, Luo, Xiao Ran, O'Donnell, Michael E, Kuriyan, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Structural Biology and Molecular Biophysics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121543/
https://www.ncbi.nlm.nih.gov/pubmed/33847559
http://dx.doi.org/10.7554/eLife.66181

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121543/
https://www.ncbi.nlm.nih.gov/pubmed/33847559
http://dx.doi.org/10.7554/eLife.66181

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