In-gel proteasome assay to determine the activity, amount, and composition of proteasome complexes from mammalian cells or tissues

This protocol describes an easy and reliable in-gel proteasome assay to quantify the activity and composition of different proteasome complexes in cells and tissues. The assay works well with limited amounts of total cell protein lysates. Although this assay is optimized specifically for the proteas...

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Detalles Bibliográficos
Autores principales: Yazgili, Ayse Seda, Meul, Thomas, Welk, Vanessa, Semren, Nora, Kammerl, Ilona E., Meiners, Silke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121766/
https://www.ncbi.nlm.nih.gov/pubmed/34027484
http://dx.doi.org/10.1016/j.xpro.2021.100526

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121766/
https://www.ncbi.nlm.nih.gov/pubmed/34027484
http://dx.doi.org/10.1016/j.xpro.2021.100526

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