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In-gel proteasome assay to determine the activity, amount, and composition of proteasome complexes from mammalian cells or tissues
This protocol describes an easy and reliable in-gel proteasome assay to quantify the activity and composition of different proteasome complexes in cells and tissues. The assay works well with limited amounts of total cell protein lysates. Although this assay is optimized specifically for the proteas...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121766/ https://www.ncbi.nlm.nih.gov/pubmed/34027484 http://dx.doi.org/10.1016/j.xpro.2021.100526 |