Production of single- and multiple-gene-modified mice via maternal SpCas9-based gene editing

Maternally and transiently accumulated SpCas9 (maternal SpCas9) in a zygote derived from a systemically SpCas9-expressing transgenic mouse strain was used to generate single- and multiple-gene-modified mice. Maternal SpCas9-based gene editing allows for high indel and knockin mutation efficiency, lo...

Descripción completa

Detalles Bibliográficos
Autores principales: Sakurai, Takayuki, Shindo, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122177/
https://www.ncbi.nlm.nih.gov/pubmed/34027476
http://dx.doi.org/10.1016/j.xpro.2021.100509

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122177/
https://www.ncbi.nlm.nih.gov/pubmed/34027476
http://dx.doi.org/10.1016/j.xpro.2021.100509

Ejemplares similares