Cargando…

Single Cell Gene Expression Analysis in a 3D Microtissue Liver Model Reveals Cell Type-Specific Responses to Pro-Fibrotic TGF-β1 Stimulation

3D cell culture systems are widely used to study disease mechanisms and therapeutic interventions. Multicellular liver microtissues (MTs) comprising HepaRG, hTERT-HSC and THP-1 maintain multicellular interactions and physiological properties required to mimic liver fibrosis. However, the inherent co...

Descripción completa

Detalles Bibliográficos
Autores principales:	Messner, Catherine Jane, Babrak, Lmar, Titolo, Gaia, Caj, Michaela, Miho, Enkelejda, Suter-Dick, Laura
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122664/ https://www.ncbi.nlm.nih.gov/pubmed/33922101 http://dx.doi.org/10.3390/ijms22094372

Ejemplares similares

Prospective Artificial Intelligence to Dissect the Dengue Immune Response and Discover Therapeutics
por: Natali, Eriberto N., et al.
Publicado: (2021)

Machine Learning Detects Anti-DENV Signatures in Antibody Repertoire Sequences
por: Horst, Alexander, et al.
Publicado: (2021)

Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis
por: Prestigiacomo, Vincenzo, et al.
Publicado: (2017)

Fibrotic microtissue array to predict anti-fibrosis drug efficacy
por: Asmani, Mohammadnabi, et al.
Publicado: (2018)

Multi-cell type human liver microtissues for hepatotoxicity testing
por: Messner, S., et al.
Publicado: (2012)

Data on the effect of pro-fibrotic cytokine TGF-β on hepatic stellate cell autophagy
por: Thomes, Paul G., et al.
Publicado: (2016)

Mycobacterium avium Possesses Extracellular DNA that Contributes to Biofilm Formation, Structural Integrity, and Tolerance to Antibiotics
por: Rose, Sasha J., et al.
Publicado: (2015)

Current strategies of mechanical stimulation for maturation of cardiac microtissues
por: Carlos-Oliveira, Maria, et al.
Publicado: (2021)

Activated Cardiac Fibroblasts Control Contraction of Human Fibrotic Cardiac Microtissues by a β-Adrenoreceptor-Dependent Mechanism
por: Błyszczuk, Przemysław, et al.
Publicado: (2020)

Pro-fibrotic SNPs
por: Maxmen, Amy
Publicado: (2009)

Benchmarking immunoinformatic tools for the analysis of antibody repertoire sequences
por: Smakaj, Erand, et al.
Publicado: (2020)

Mycobacterium tuberculosis Alters the Metalloprotease Activity of the COP9 Signalosome
por: Danelishvili, Lia, et al.
Publicado: (2014)

Rapid Microfluidic Assay for the Detection of Botulinum Neurotoxin in Animal Sera
por: Babrak, Lmar, et al.
Publicado: (2016)

Correction to: Current strategies of mechanical stimulation for maturation of cardiac microtissues
por: Carlos-Oliveira, Maria, et al.
Publicado: (2022)

Excess TGF-β1 Drives Cardiac Mesenchymal Stromal Cells to a Pro-Fibrotic Commitment in Arrhythmogenic Cardiomyopathy
por: Maione, Angela Serena, et al.
Publicado: (2021)

Nanofabrication of Nonfouling Surfaces for Micropatterning of Cell and Microtissue
por: Otsuka, Hidenori
Publicado: (2010)

RWD-Cockpit: Application for Quality Assessment of Real-world Data
por: Babrak, Lmar Marie, et al.
Publicado: (2022)

Anti-diabetic treatment regulates pro-fibrotic TGF-β serum levels in type 2 diabetics
por: Pscherer, Stefan, et al.
Publicado: (2013)

Fibulin-6 regulates pro-fibrotic TGF-β responses in neonatal mouse ventricular cardiac fibroblasts
por: Chowdhury, Arpita, et al.
Publicado: (2017)

Presence of Virulence-Associated Genes and Ability to Form Biofilm among Clinical Isolates of Escherichia coli Causing Urinary Infection in Domestic Animals
por: Hill, Cherise, et al.
Publicado: (2015)

Waffle‐inspired hydrogel‐based macrodevice for spatially controlled distribution of encapsulated therapeutic microtissues and pro‐angiogenic endothelial cells
por: Pham, Chi H. L., et al.
Publicado: (2023)

The pro-fibrotic and anti-inflammatory foam cell macrophage paradox
por: Thomas, Anita C., et al.
Publicado: (2015)

Using the Microwell-mesh to culture microtissues in vitro and as a carrier to implant microtissues in vivo into mice
por: Monterosso, Melissa E., et al.
Publicado: (2021)

Pro-Fibrotic Phenotype in a Patient with Segmental Stiff Skin Syndrome via TGF-β Signaling Overactivation
por: Fusco, Carmela, et al.
Publicado: (2020)

TGF-β1 Induces Interlukin-11 Expression and Pro-Fibrotic Effect by DNA Demethylation in Subconjunctival Fibroblasts
por: Fu, Shuhao, et al.
Publicado: (2022)

Increased Cell Traction-Induced Prestress in Dynamically Cultured Microtissues
por: van Kelle, Mathieu A. J., et al.
Publicado: (2019)

Transcriptomic, Proteomic, and Functional Long-Term Characterization of Multicellular Three-Dimensional Human Liver Microtissues
por: Messner, Simon, et al.
Publicado: (2018)

Identification of Mycobacterium avium subsp. hominissuis secreted proteins using an in vitro system mimicking the phagosomal environment
por: Chinison, Jessica J., et al.
Publicado: (2016)

Infiltration of immune cells into cancer cell/stroma cell 3D microtissues
por: Koeck, Stefan, et al.
Publicado: (2015)

Utility of spherical human liver microtissues for prediction of clinical drug-induced liver injury
por: Proctor, William R., et al.
Publicado: (2017)

Extracellular SPARC cooperates with TGF-β signalling to induce pro-fibrotic activation of systemic sclerosis patient dermal fibroblasts
por: Carvalheiro, Tiago, et al.
Publicado: (2020)

Retracted: TGF-β1 Induces Interlukin-11 Expression and Pro-Fibrotic Effect by DNA Demethylation in Subconjunctival Fibroblasts
por: and Alternative Medicine, Evidence-Based Complementary
Publicado: (2023)

Method for manufacture and cryopreservation of cartilage microtissues
por: Shajib, Md. Shafiullah, et al.
Publicado: (2023)

Methotrexate-Induced Liver Injury Is Associated with Oxidative Stress, Impaired Mitochondrial Respiration, and Endoplasmic Reticulum Stress In Vitro
por: Schmidt, Saskia, et al.
Publicado: (2022)

Periostin—An inducer of pro-fibrotic phenotype in monocytes and monocyte-derived macrophages in systemic sclerosis
por: Suzuki, Mao, et al.
Publicado: (2023)

Pan-Phosphodiesterase Inhibitors Attenuate TGF-β-Induced Pro-Fibrotic Phenotype in Alveolar Epithelial Type II Cells by Downregulating Smad-2 Phosphorylation
por: Wójcik-Pszczoła, Katarzyna, et al.
Publicado: (2022)

Anti-fibrotic potential of erythropoietin signaling on bone marrow derived fibrotic cell
por: Iwata, Yasunori, et al.
Publicado: (2021)

Functional arrays of human pluripotent stem cell-derived cardiac microtissues
por: Thavandiran, Nimalan, et al.
Publicado: (2020)

Development and Angiogenic Potential of Cell-Derived Microtissues Using Microcarrier-Template
por: Rubí-Sans, Gerard, et al.
Publicado: (2021)

Chondrogenic Potential of Human Dental Pulp Stem Cells Cultured as Microtissues
por: Salvador-Clavell, Rubén, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_umcd9v34gi20jdoc87tamkdqlk