A Cytopathic Effect-Based Tissue Culture Method for HCoV-OC43 Titration Using TMPRSS2-Expressing VeroE6 Cells

Human coronavirus (HCoV)-OC43 rarely shows a cytopathic effect (CPE) after infection of various cell lines, and the indirect immunoperoxidase assay (IPA), a relatively complex procedure, has long been used as an alternative assay. Because HCoV-OC43 uses cell-surface transmembrane protease serine 2 (...

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Detalles Bibliográficos
Autores principales: Hirose, Ryohei, Watanabe, Naoto, Bandou, Risa, Yoshida, Takuma, Daidoji, Tomo, Naito, Yuji, Itoh, Yoshito, Nakaya, Takaaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125049/
https://www.ncbi.nlm.nih.gov/pubmed/33980675
http://dx.doi.org/10.1128/mSphere.00159-21

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125049/
https://www.ncbi.nlm.nih.gov/pubmed/33980675
http://dx.doi.org/10.1128/mSphere.00159-21

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