Cargando…
Biomechanical-Based Protocol for in vitro Study of Cartilage Response to Cyclic Loading: A Proof-of-Concept in Knee Osteoarthritis
Osteoarthritis (OA) is an evolving disease and a major cause of pain and impaired mobility. A deeper understanding of cartilage metabolism in response to loading is critical to achieve greater insight into OA mechanisms. While physiological joint loading helps maintain cartilage integrity, reduced o...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126668/ https://www.ncbi.nlm.nih.gov/pubmed/34012954 http://dx.doi.org/10.3389/fbioe.2021.634327 |
_version_ | 1783693808860397568 |
---|---|
author | Caravaggi, Paolo Assirelli, Elisa Ensini, Andrea Ortolani, Maurizio Mariani, Erminia Leardini, Alberto Neri, Simona Belvedere, Claudio |
author_facet | Caravaggi, Paolo Assirelli, Elisa Ensini, Andrea Ortolani, Maurizio Mariani, Erminia Leardini, Alberto Neri, Simona Belvedere, Claudio |
author_sort | Caravaggi, Paolo |
collection | PubMed |
description | Osteoarthritis (OA) is an evolving disease and a major cause of pain and impaired mobility. A deeper understanding of cartilage metabolism in response to loading is critical to achieve greater insight into OA mechanisms. While physiological joint loading helps maintain cartilage integrity, reduced or excessive loading have catabolic effects. The main scope of this study is to present an original methodology potentially capable to elucidate the effect of cyclic joint loading on cartilage metabolism, to identify mechanisms involved in preventing or slowing down OA progression, and to provide preliminary data on its application. In the proposed protocol, the combination of biomechanical data and medical imaging are integrated with molecular information about chondrocyte mechanotransduction and tissue homeostasis. The protocol appears to be flexible and suitable to analyze human OA knee cartilage explants, with different degrees of degeneration, undergoing ex vivo realistic cyclic joint loading estimated via gait analysis in patients simulating mild activities of daily living. The modulation of molecules involved in cartilage homeostasis, mechanotransduction, inflammation, pain and wound healing can be analyzed in chondrocytes and culture supernatants. A thorough analysis performed with the proposed methodology, combining in vivo functional biomechanical evaluations with ex vivo molecular assessments is expected to provide new insights on the beneficial effects of physiological loading and contribute to the design and optimization of non-pharmacological treatments limiting OA progression. |
format | Online Article Text |
id | pubmed-8126668 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81266682021-05-18 Biomechanical-Based Protocol for in vitro Study of Cartilage Response to Cyclic Loading: A Proof-of-Concept in Knee Osteoarthritis Caravaggi, Paolo Assirelli, Elisa Ensini, Andrea Ortolani, Maurizio Mariani, Erminia Leardini, Alberto Neri, Simona Belvedere, Claudio Front Bioeng Biotechnol Bioengineering and Biotechnology Osteoarthritis (OA) is an evolving disease and a major cause of pain and impaired mobility. A deeper understanding of cartilage metabolism in response to loading is critical to achieve greater insight into OA mechanisms. While physiological joint loading helps maintain cartilage integrity, reduced or excessive loading have catabolic effects. The main scope of this study is to present an original methodology potentially capable to elucidate the effect of cyclic joint loading on cartilage metabolism, to identify mechanisms involved in preventing or slowing down OA progression, and to provide preliminary data on its application. In the proposed protocol, the combination of biomechanical data and medical imaging are integrated with molecular information about chondrocyte mechanotransduction and tissue homeostasis. The protocol appears to be flexible and suitable to analyze human OA knee cartilage explants, with different degrees of degeneration, undergoing ex vivo realistic cyclic joint loading estimated via gait analysis in patients simulating mild activities of daily living. The modulation of molecules involved in cartilage homeostasis, mechanotransduction, inflammation, pain and wound healing can be analyzed in chondrocytes and culture supernatants. A thorough analysis performed with the proposed methodology, combining in vivo functional biomechanical evaluations with ex vivo molecular assessments is expected to provide new insights on the beneficial effects of physiological loading and contribute to the design and optimization of non-pharmacological treatments limiting OA progression. Frontiers Media S.A. 2021-05-03 /pmc/articles/PMC8126668/ /pubmed/34012954 http://dx.doi.org/10.3389/fbioe.2021.634327 Text en Copyright © 2021 Caravaggi, Assirelli, Ensini, Ortolani, Mariani, Leardini, Neri and Belvedere. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Caravaggi, Paolo Assirelli, Elisa Ensini, Andrea Ortolani, Maurizio Mariani, Erminia Leardini, Alberto Neri, Simona Belvedere, Claudio Biomechanical-Based Protocol for in vitro Study of Cartilage Response to Cyclic Loading: A Proof-of-Concept in Knee Osteoarthritis |
title | Biomechanical-Based Protocol for in vitro Study of Cartilage Response to Cyclic Loading: A Proof-of-Concept in Knee Osteoarthritis |
title_full | Biomechanical-Based Protocol for in vitro Study of Cartilage Response to Cyclic Loading: A Proof-of-Concept in Knee Osteoarthritis |
title_fullStr | Biomechanical-Based Protocol for in vitro Study of Cartilage Response to Cyclic Loading: A Proof-of-Concept in Knee Osteoarthritis |
title_full_unstemmed | Biomechanical-Based Protocol for in vitro Study of Cartilage Response to Cyclic Loading: A Proof-of-Concept in Knee Osteoarthritis |
title_short | Biomechanical-Based Protocol for in vitro Study of Cartilage Response to Cyclic Loading: A Proof-of-Concept in Knee Osteoarthritis |
title_sort | biomechanical-based protocol for in vitro study of cartilage response to cyclic loading: a proof-of-concept in knee osteoarthritis |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126668/ https://www.ncbi.nlm.nih.gov/pubmed/34012954 http://dx.doi.org/10.3389/fbioe.2021.634327 |
work_keys_str_mv | AT caravaggipaolo biomechanicalbasedprotocolforinvitrostudyofcartilageresponsetocyclicloadingaproofofconceptinkneeosteoarthritis AT assirellielisa biomechanicalbasedprotocolforinvitrostudyofcartilageresponsetocyclicloadingaproofofconceptinkneeosteoarthritis AT ensiniandrea biomechanicalbasedprotocolforinvitrostudyofcartilageresponsetocyclicloadingaproofofconceptinkneeosteoarthritis AT ortolanimaurizio biomechanicalbasedprotocolforinvitrostudyofcartilageresponsetocyclicloadingaproofofconceptinkneeosteoarthritis AT marianierminia biomechanicalbasedprotocolforinvitrostudyofcartilageresponsetocyclicloadingaproofofconceptinkneeosteoarthritis AT leardinialberto biomechanicalbasedprotocolforinvitrostudyofcartilageresponsetocyclicloadingaproofofconceptinkneeosteoarthritis AT nerisimona biomechanicalbasedprotocolforinvitrostudyofcartilageresponsetocyclicloadingaproofofconceptinkneeosteoarthritis AT belvedereclaudio biomechanicalbasedprotocolforinvitrostudyofcartilageresponsetocyclicloadingaproofofconceptinkneeosteoarthritis |