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Data on the optimization of an archaea-specific probe-based qPCR assay

Estimation of archaeal numbers by use of fluorescent DNA binding dyes is challenging, because primers targeting the archaeal 16SrRNA genes readily also bind to bacterial 16S rRNA gene sequences, especially when the relative abundance of bacteria is greater than that of archaea. In order to increase...

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Detalles Bibliográficos
Autores principales: Bomberg, Malin, Miettinen, Hanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129643/
https://www.ncbi.nlm.nih.gov/pubmed/34026962
http://dx.doi.org/10.1016/j.dib.2020.106610