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Measurement of mitochondrial respiration in the murine retina using a Seahorse extracellular flux analyzer

Mitochondrial metabolism is a critical mechanism that is deregulated in numerous retinal diseases. Here, we elaborate a protocol to quantify oxygen consumption rate as a measure of mitochondrial respiration directly from mouse retinal tissue pieces. Our procedure combines the use of Seahorse extrace...

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Detalles Bibliográficos
Autores principales: Shetty, Trupti, Park, Bomina, Corson, Timothy W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132104/
https://www.ncbi.nlm.nih.gov/pubmed/34027490
http://dx.doi.org/10.1016/j.xpro.2021.100533