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Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors

Tumor organoids and patient-derived orthotopic xenografts (PDOXs) are some of the most valuable pre-clinical tools in cancer research. In this protocol, we describe efficient derivation of organoids and PDOX models from glioma patient tumors. We provide detailed steps for organoid culture, intracran...

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Autores principales: Oudin, Anaïs, Baus, Virginie, Barthelemy, Vanessa, Fabian, Carina, Klein, Eliane, Dieterle, Monika, Wantz, May, Hau, Ann-Christin, Dording, Claire, Bernard, Amandine, Michelucci, Alessandro, Yabo, Yahaya A., Kanli, Georgia, Keunen, Olivier, Bjerkvig, Rolf, Niclou, Simone P., Golebiewska, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132120/
https://www.ncbi.nlm.nih.gov/pubmed/34027491
http://dx.doi.org/10.1016/j.xpro.2021.100534
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author Oudin, Anaïs
Baus, Virginie
Barthelemy, Vanessa
Fabian, Carina
Klein, Eliane
Dieterle, Monika
Wantz, May
Hau, Ann-Christin
Dording, Claire
Bernard, Amandine
Michelucci, Alessandro
Yabo, Yahaya A.
Kanli, Georgia
Keunen, Olivier
Bjerkvig, Rolf
Niclou, Simone P.
Golebiewska, Anna
author_facet Oudin, Anaïs
Baus, Virginie
Barthelemy, Vanessa
Fabian, Carina
Klein, Eliane
Dieterle, Monika
Wantz, May
Hau, Ann-Christin
Dording, Claire
Bernard, Amandine
Michelucci, Alessandro
Yabo, Yahaya A.
Kanli, Georgia
Keunen, Olivier
Bjerkvig, Rolf
Niclou, Simone P.
Golebiewska, Anna
author_sort Oudin, Anaïs
collection PubMed
description Tumor organoids and patient-derived orthotopic xenografts (PDOXs) are some of the most valuable pre-clinical tools in cancer research. In this protocol, we describe efficient derivation of organoids and PDOX models from glioma patient tumors. We provide detailed steps for organoid culture, intracranial implantation, and detection of tumors in the brain. We further present technical adjustments for standardized functional assays and drug testing. For complete details on the use and execution of this protocol, please refer to Golebiewska et al. (2020).
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spelling pubmed-81321202021-05-21 Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors Oudin, Anaïs Baus, Virginie Barthelemy, Vanessa Fabian, Carina Klein, Eliane Dieterle, Monika Wantz, May Hau, Ann-Christin Dording, Claire Bernard, Amandine Michelucci, Alessandro Yabo, Yahaya A. Kanli, Georgia Keunen, Olivier Bjerkvig, Rolf Niclou, Simone P. Golebiewska, Anna STAR Protoc Protocol Tumor organoids and patient-derived orthotopic xenografts (PDOXs) are some of the most valuable pre-clinical tools in cancer research. In this protocol, we describe efficient derivation of organoids and PDOX models from glioma patient tumors. We provide detailed steps for organoid culture, intracranial implantation, and detection of tumors in the brain. We further present technical adjustments for standardized functional assays and drug testing. For complete details on the use and execution of this protocol, please refer to Golebiewska et al. (2020). Elsevier 2021-05-12 /pmc/articles/PMC8132120/ /pubmed/34027491 http://dx.doi.org/10.1016/j.xpro.2021.100534 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Oudin, Anaïs
Baus, Virginie
Barthelemy, Vanessa
Fabian, Carina
Klein, Eliane
Dieterle, Monika
Wantz, May
Hau, Ann-Christin
Dording, Claire
Bernard, Amandine
Michelucci, Alessandro
Yabo, Yahaya A.
Kanli, Georgia
Keunen, Olivier
Bjerkvig, Rolf
Niclou, Simone P.
Golebiewska, Anna
Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
title Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
title_full Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
title_fullStr Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
title_full_unstemmed Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
title_short Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
title_sort protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132120/
https://www.ncbi.nlm.nih.gov/pubmed/34027491
http://dx.doi.org/10.1016/j.xpro.2021.100534
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