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HCK and ABAA: A Newly Designed Pipeline to Improve Fungi Metabarcoding Analysis
INTRODUCTION: The fungi ITS sequence length dissimilarity, non-specific amplicons, including chimaera formed during Polymerase Chain Reaction (PCR), added to sequencing errors, create bias during similarity clustering and abundance estimation in the downstream analysis. To overcome these challenges,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134036/ https://www.ncbi.nlm.nih.gov/pubmed/34025601 http://dx.doi.org/10.3389/fmicb.2021.640693 |