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Quantitative evaluation of macromolecular crowding environment based on translational and rotational diffusion using polarization dependent fluorescence correlation spectroscopy

Macromolecular crowding (MMC) in cells is a hot topic in biology; therefore, well-characterized measurement standards for the evaluation of the nano-environment in MMC solutions are necessary. We propose to use polarization-dependent fluorescence correlation spectroscopy (Pol-FCS) for evaluation of...

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Detalles Bibliográficos
Autores principales: Yamamoto, Johtaro, Matsui, Akito, Gan, Fusako, Oura, Makoto, Ando, Riku, Matsuda, Takahiro, Gong, Jian Ping, Kinjo, Masataka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134472/
https://www.ncbi.nlm.nih.gov/pubmed/34011998
http://dx.doi.org/10.1038/s41598-021-89987-7