Expression and purification of soluble and active human enterokinase light chain in Escherichia coli

Human enterokinase light chain (hEK(L)) specifically cleaves the sequence (Asp)(4)-Lys↓X (D(4)K), making this a frequently used enzyme for site-specific cleavage of recombinant fusion proteins. However, hEK(L) production from Escherichia coli is limited due to intramolecular disulphide bonds. Here,...

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Detalles Bibliográficos
Autores principales: Kim, Young Su, Lee, Hye-Jeong, Park, Sang-hyun, Kim, Yeu-chun, Ahn, Jungoh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134707/
https://www.ncbi.nlm.nih.gov/pubmed/34026576
http://dx.doi.org/10.1016/j.btre.2021.e00626

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134707/
https://www.ncbi.nlm.nih.gov/pubmed/34026576
http://dx.doi.org/10.1016/j.btre.2021.e00626

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