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Expression and purification of soluble and active human enterokinase light chain in Escherichia coli

Human enterokinase light chain (hEK(L)) specifically cleaves the sequence (Asp)(4)-Lys↓X (D(4)K), making this a frequently used enzyme for site-specific cleavage of recombinant fusion proteins. However, hEK(L) production from Escherichia coli is limited due to intramolecular disulphide bonds. Here,...

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Detalles Bibliográficos
Autores principales:	Kim, Young Su, Lee, Hye-Jeong, Park, Sang-hyun, Kim, Yeu-chun, Ahn, Jungoh
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Short Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134707/ https://www.ncbi.nlm.nih.gov/pubmed/34026576 http://dx.doi.org/10.1016/j.btre.2021.e00626

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