Decoupling the bridge helix of Cas12a results in a reduced trimming activity, increased mismatch sensitivity and impaired conformational transitions

The widespread and versatile prokaryotic CRISPR–Cas systems (clustered regularly interspaced short palindromic repeats and associated Cas proteins) constitute powerful weapons against foreign nucleic acids. Recently, the single-effector nuclease Cas12a that belongs to the type V CRISPR–Cas system wa...

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Detalles Bibliográficos
Autores principales: Wörle, Elisabeth, Jakob, Leonhard, Schmidbauer, Andreas, Zinner, Gabriel, Grohmann, Dina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136826/
https://www.ncbi.nlm.nih.gov/pubmed/34009379
http://dx.doi.org/10.1093/nar/gkab286

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136826/
https://www.ncbi.nlm.nih.gov/pubmed/34009379
http://dx.doi.org/10.1093/nar/gkab286

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