Implementation of dCas9-mediated CRISPRi in the fission yeast Schizosaccharomyces pombe

Controllable and reversible transcriptional repression is an essential method to study gene functions. A systematic knock-down method using catalytically inactive Cas9 (dCas9) was originally established in bacteria. dCas9 forms a ribonucleoprotein with a small guide RNA and uses it to recognize a sp...

Descripción completa

Detalles Bibliográficos
Autores principales: Ishikawa, Ken, Soejima, Saeko, Masuda, Fumie, Saitoh, Shigeaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Investigation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137136/
https://www.ncbi.nlm.nih.gov/pubmed/33617628
http://dx.doi.org/10.1093/g3journal/jkab051

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137136/
https://www.ncbi.nlm.nih.gov/pubmed/33617628
http://dx.doi.org/10.1093/g3journal/jkab051

Ejemplares similares