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Implementation of dCas9-mediated CRISPRi in the fission yeast Schizosaccharomyces pombe
Controllable and reversible transcriptional repression is an essential method to study gene functions. A systematic knock-down method using catalytically inactive Cas9 (dCas9) was originally established in bacteria. dCas9 forms a ribonucleoprotein with a small guide RNA and uses it to recognize a sp...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137136/ https://www.ncbi.nlm.nih.gov/pubmed/33617628 http://dx.doi.org/10.1093/g3journal/jkab051 |