Cargando…

An emm-type specific qPCR to track bacterial load during experimental human Streptococcus pyogenes pharyngitis

BACKGROUND: Streptococcus pyogenes causes a profound global burden of morbidity and mortality across its diverse clinical spectrum. To support a new controlled human infection (challenge) model seeking to accelerate S. pyogenes vaccine development, we aimed to develop an accurate and reliable molecu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fabri, Loraine V., Azzopardi, Kristy I., Osowicki, Joshua, Frost, Hannah R., Smeesters, Pierre R., Steer, Andrew C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2021
Materias:	Technical Advance
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138111/ https://www.ncbi.nlm.nih.gov/pubmed/34020607 http://dx.doi.org/10.1186/s12879-021-06173-w

Ejemplares similares

Detection of group a streptococcal pharyngitis by quantitative PCR
por: Dunne, Eileen M, et al.
Publicado: (2013)

Immune signature of acute pharyngitis in a Streptococcus pyogenes human challenge trial
por: Anderson, Jeremy, et al.
Publicado: (2022)

A Controlled Human Infection Model of Group A Streptococcus Pharyngitis: Which Strain and Why?
por: Osowicki, Joshua, et al.
Publicado: (2019)

Development and Validation of Two RT-qPCR Diagnostic Assays for Detecting Severe Acute Respiratory Syndrome Coronavirus 2 Genomic Targets across Two Specimen Types
por: Miller, Eric W., et al.
Publicado: (2022)

Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M. leprae from nasal swab samples
por: Beissner, Marcus, et al.
Publicado: (2019)

Sequential multiplex PCR assay for determining capsular serotypes of colonizing S. pneumoniae
por: Jourdain, Sarah, et al.
Publicado: (2011)

SISH/CISH or qPCR as alternative techniques to FISH for determination of HER2 amplification status on breast tumors core needle biopsies: a multicenter experience based on 840 cases
por: Jacquemier, Jocelyne, et al.
Publicado: (2013)

Added Value of the emm-Cluster Typing System to Analyze Group A Streptococcus Epidemiology in High-Income Settings
por: Shulman, Stanford T., et al.
Publicado: (2014)

Comparative M-protein analysis of Streptococcus pyogenes from pharyngitis and skin infections in New Zealand: Implications for vaccine development
por: Williamson, Deborah A, et al.
Publicado: (2016)

Complete Genome Sequence of Hypervirulent Streptococcus pyogenes emm3 Strain 1838
por: Lei, Benfang, et al.
Publicado: (2019)

Validation of an automated colony counting system for group A Streptococcus
por: Frost, H. R., et al.
Publicado: (2016)

Cryptic prophages within a Streptococcus pyogenes genotype emm4 lineage
por: Remmington, Alex, et al.
Publicado: (2020)

Maximising the Use of Scarce qPCR Master Mixes
por: Bustin, Stephen, et al.
Publicado: (2022)

Detection of six soil-transmitted helminths in human stool by qPCR- a systematic workflow
por: Azzopardi, Kristy I., et al.
Publicado: (2021)

Technical validation of an RT-qPCR in vitro diagnostic test system for the determination of breast cancer molecular subtypes by quantification of ERBB2, ESR1, PGR and MKI67 mRNA levels from formalin-fixed paraffin-embedded breast tumor specimens
por: Laible, Mark, et al.
Publicado: (2016)

Susceptibility and emm type of Streptococcus pyogenes isolated from children with severe infection
por: Sakata, Hiroshi
Publicado: (2013)

Group A Streptococcus virulence factors genes in north India & their association with emm type in pharyngitis
por: Dhanda, V., et al.
Publicado: (2011)

Group A Streptococcus emm Gene Types in Pharyngeal Isolates, Ontario, Canada, 2002–2010
por: Shea, Patrick R., et al.
Publicado: (2011)

Modeling Streptococcus pyogenes Pharyngeal Colonization in the Mouse
por: Gogos, Artemis, et al.
Publicado: (2019)

Antimicrobial susceptibility patterns, emm type distribution and genetic diversity of Streptococcus pyogenes recovered in Brazil
por: Arêas, Glauber P, et al.
Publicado: (2014)

Clonal Spread of Streptococcus pyogenes emm44 among Homeless Persons, Rennes, France
por: Cady, Anne, et al.
Publicado: (2011)

Spread of a highly mucoid Streptococcus pyogenes emm3/ST15 clone
por: Tamayo, Esther, et al.
Publicado: (2010)

Complete Genome Sequence of emm Type 14 Streptococcus pyogenes Strain HSC5
por: Port, Gary C., et al.
Publicado: (2013)

Whole-Genome Sequence of Streptococcus pyogenes Strain 591, Belonging to the Genotype emm49
por: Patenge, Nadja, et al.
Publicado: (2021)

Streptococcus pyogenes emm98.1 variants activate inflammatory caspases in human neutrophils
por: Williams, Jonathan G., et al.
Publicado: (2023)

Relationships between emm and multilocus sequence types within a global collection of Streptococcus pyogenes
por: Bessen, Debra E, et al.
Publicado: (2008)

Closed Genome Sequence of Noninvasive Streptococcus pyogenes M/emm3 Strain STAB902
por: Soriano, Nicolas, et al.
Publicado: (2014)

Changes in emm types and superantigen gene content of Streptococcus pyogenes causing invasive infections in Portugal
por: Friães, A., et al.
Publicado: (2019)

Genetic Characterization of Streptococcus pyogenes emm89 Strains Isolated in Japan From 2011 to 2019
por: Hirose, Yujiro, et al.
Publicado: (2020)

Streptococcus pyogenes Lineage ST62/emm87: The International Spread of This Potentially Invasive Lineage
por: Martini, Caroline Lopes, et al.
Publicado: (2023)

Superantigen profiles of emm and emm-like typeable and nontypeable pharyngeal streptococcal isolates of South India
por: Anand, Thangarajan Durai, et al.
Publicado: (2012)

Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness
por: Szewczuk, Elektra, et al.
Publicado: (2010)

Contemporary Pharyngeal and Invasive emm1 and Invasive emm12 Group A Streptococcus Isolates Exhibit Similar In Vivo Selection for CovRS Mutants in Mice
por: Feng, Wenchao, et al.
Publicado: (2016)

Development of a quadruple qRT-PCR assay for simultaneous identification of highly and low pathogenic H7N9 avian influenza viruses and characterization against oseltamivir resistance
por: Yang, Yang, et al.
Publicado: (2018)

Complete Genome Sequences of emm111 Type Streptococcus pyogenes Strain GUR, with Antitumor Activity, and Its Derivative Strain GURSA1 with an Inactivated emm Gene
por: Suvorova, Maria A., et al.
Publicado: (2017)

Complete Genome Sequences of emm6 Streptococcus pyogenes JRS4 and Parental Strain D471
por: Port, Gary C., et al.
Publicado: (2015)

Genome sequence analysis of emm89 Streptococcus pyogenes strains causing infections in Scotland, 2010–2016
por: Beres, Stephen B., et al.
Publicado: (2017)

Comparison of nested PCR and real time PCR of Herpesvirus infections of central nervous system in HIV patients
por: Drago, Lorenzo, et al.
Publicado: (2004)

PCR amplification of a triple-repeat genetic target directly from whole blood in 15 minutes as a proof-of-principle PCR study for direct sample analysis for a clinically relevant target
por: Connelly, Christopher M, et al.
Publicado: (2014)

The ‘PREXCEL-Q Method’ for qPCR
por: Gallup, Jack M., et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_hg853than7ampg6qmvq4jlduvd