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In vitro co-culture of human intestinal organoids and lamina propria-derived CD4(+) T cells
Crosstalk between immune cells and intestinal stem cells (ISCs) in vivo plays a critical role in tissue homeostasis and inflammation; however, in vitro models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous in vitro long-ter...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138864/ https://www.ncbi.nlm.nih.gov/pubmed/34036282 http://dx.doi.org/10.1016/j.xpro.2021.100519 |