In vitro co-culture of human intestinal organoids and lamina propria-derived CD4(+) T cells

Crosstalk between immune cells and intestinal stem cells (ISCs) in vivo plays a critical role in tissue homeostasis and inflammation; however, in vitro models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous in vitro long-ter...

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Detalles Bibliográficos
Autores principales: Schreurs, Renée R.C.E., Baumdick, Martin E., Drewniak, Agata, Bunders, Madeleine J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138864/
https://www.ncbi.nlm.nih.gov/pubmed/34036282
http://dx.doi.org/10.1016/j.xpro.2021.100519
Descripción
Sumario:Crosstalk between immune cells and intestinal stem cells (ISCs) in vivo plays a critical role in tissue homeostasis and inflammation; however, in vitro models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous in vitro long-term 3D co-culture system of human intestinal CD4(+) T cells and ISCs to study T cell-intestinal epithelial cell interactions during tissue development and inflammation. For complete details on the use and execution of this protocol, please refer to Schreurs et al. (2019).

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