Cargando…

In vitro co-culture of human intestinal organoids and lamina propria-derived CD4(+) T cells

Crosstalk between immune cells and intestinal stem cells (ISCs) in vivo plays a critical role in tissue homeostasis and inflammation; however, in vitro models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous in vitro long-ter...

Descripción completa

Detalles Bibliográficos
Autores principales:	Schreurs, Renée R.C.E., Baumdick, Martin E., Drewniak, Agata, Bunders, Madeleine J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138864/ https://www.ncbi.nlm.nih.gov/pubmed/34036282 http://dx.doi.org/10.1016/j.xpro.2021.100519

Ejemplares similares

Isolation and analyses of lamina propria lymphocytes from mouse intestines
por: Kim, Eunha, et al.
Publicado: (2022)

Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells
por: Benmeziane, Keltouma, et al.
Publicado: (2022)

Characterizing CD4 T cell differentiation in mouse small intestine using T cell transfer, lamina propria preparation, and flow cytometry
por: Duan, Jinzhi, et al.
Publicado: (2023)

Chimerism and phenotypic analysis of intraepithelial and lamina propria T cells isolated from human ileal biopsies after intestinal transplantation
por: Long, Katherine D., et al.
Publicado: (2023)

Thiol-Mediated Redox Regulation of Intestinal Lamina Propria T Lymphocytes
por: Sido, Bernd, et al.
Publicado: (2000)

Dendritic cell subsets in the intestinal lamina propria: Ontogeny and function
por: Persson, Emma K, et al.
Publicado: (2013)

Immunoregulation by antigen-presenting cells in human intestinal lamina propria
por: Ogino, Takayuki, et al.
Publicado: (2023)

The Neonatal Development of Intraepithelial and Lamina Propria Lymphocytes in the Murine Small Intestine
por: Steege, Jessica C. A. Ter, et al.
Publicado: (1997)

Characteristics of natural killer cells in the murine intestinal epithelium and lamina propria
Publicado: (1982)

Isolation and characterization of lymphocytes from bovine intestinal epithelium and lamina propria
por: Clough, Ellen R., et al.
Publicado: (1988)

Microbiota of the Small Intestine Is Selectively Engulfed by Phagocytes of the Lamina Propria and Peyer’s Patches
por: Morikawa, Masatoshi, et al.
Publicado: (2016)

Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism
por: Welty, Nathan E., et al.
Publicado: (2013)

Initiation of an Inflammatory Response in Resident Intestinal Lamina Propria Cells -Use of a Human Organ Culture Model
por: Schröder-Braunstein, Jutta, et al.
Publicado: (2014)

Synergy Between Intraepithelial Lymphocytes and Lamina Propria T Cells Drives Intestinal Inflammation During Infection
por: Egan, C. E., et al.
Publicado: (2011)

Disturbances in apoptosis of lamina propria lymphocytes in Crohn's disease
por: Eder, Piotr, et al.
Publicado: (2015)

Increased calcium channel in the lamina propria of aging rat
por: Kim, Ji Min, et al.
Publicado: (2019)

Sulfated glycosaminoglycans in human vocal fold lamina propria()
por: Park, Sung Woo, et al.
Publicado: (2016)

Intestinal organoid co-culture protocol to study cell competition in vitro
por: van Neerven, Sanne M., et al.
Publicado: (2021)

TLR5 mediates CD172α(+) intestinal lamina propria dendritic cell induction of Th17 cells
por: Liu, Han, et al.
Publicado: (2016)

Extracellular vesicles derived from small intestinal lamina propria reduce antigen-specific immune response
por: Shin, Tae-Seop, et al.
Publicado: (2022)

Correction: Initiation of an Inflammatory Response in Resident Intestinal Lamina Propria Cells -Use of a Human Organ Culture Model
Publicado: (2014)

Signal regulatory protein alpha (SIRPα) regulates the homeostasis of CD103(+)CD11b(+) DCs in the intestinal lamina propria
por: Scott, Charlotte L, et al.
Publicado: (2014)

Stimulation of lamina propria lymphocytes by intestinal epithelial cells: evidence for recognition of nonclassical restriction elements
Publicado: (1994)

CTLA-4 promotes Foxp3 induction and regulatory T cell accumulation in the intestinal lamina propria
por: Barnes, M J, et al.
Publicado: (2013)

Isolation and functional characterisation of lamina propria leukocytes from helminth-infected, murine small intestine
por: Webster, Holly C., et al.
Publicado: (2020)

Some Gammaproteobacteria are enriched within CD14(+) macrophages from intestinal lamina propria of Crohn’s disease patients versus mucus
por: Sekido, Yuki, et al.
Publicado: (2020)

Luminal DMSO: Effects on Detrusor and Urothelial/Lamina Propria Function
por: Smith, Katrina J., et al.
Publicado: (2014)

Identification of telocytes in the upper lamina propria of the human urinary tract
por: Gevaert, Thomas, et al.
Publicado: (2012)

Microbiota Controls the Homeostasis of Glial Cells in the Gut Lamina Propria
por: Kabouridis, Panagiotis S., et al.
Publicado: (2015)

Immunocytes, Enterocytes and the Lamina Propria: An Immunopathological Framework of Coeliac Disease
por: Marsh, M. N.
Publicado: (1983)

Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease
por: Elliott, Timothy R., et al.
Publicado: (2015)

Irgm1-deficiency leads to myeloid dysfunction in colon lamina propria and susceptibility to the intestinal pathogen Citrobacter rodentium
por: Taylor, Gregory A., et al.
Publicado: (2020)

Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine
por: Ochi, Takanori, et al.
Publicado: (2016)

Identification of telocytes in the lamina propria of rat duodenum: transmission electron microscopy
por: Carmona, I Cantarero, et al.
Publicado: (2011)

Telocytes in gastric lamina propria of the Chinese giant salamander, Andrias davidianus
por: Zhang, Hui, et al.
Publicado: (2016)

Establishment and Culture of Human Intestinal Organoids Derived from Adult Stem Cells
por: Pleguezuelos‐Manzano, Cayetano, et al.
Publicado: (2020)

Disentangling the innate immune responses of intestinal epithelial cells and lamina propria cells to Salmonella Typhimurium infection in chickens
por: Sutton, Kate, et al.
Publicado: (2023)

Microbial colonization influences early B-lineage development in the gut lamina propria
por: Wesemann, Duane R., et al.
Publicado: (2013)

Rhythmic Calcium Events in the Lamina Propria Network of the Urinary Bladder of Rat Pups
por: Heppner, Thomas J., et al.
Publicado: (2017)

Intraepithelial and Lamina Propria Lymphocytes Do Not Correlate With Symptoms or Exposures in Microscopic Colitis
por: Sandler, Robert S., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_6lgmjdigr5dn7m5idjd040sv82