Evaluating the cleavage efficacy of CRISPR-Cas9 sgRNAs targeting ineffective regions of Arabidopsis thaliana genome

The CRISPR-Cas9 system has recently evolved as a powerful mutagenic tool for targeted genome editing. The impeccable functioning of the system depends on the optimal design of single guide RNAs (sgRNAs) that mainly involves sgRNA specificity and on-target cleavage efficacy. Several research groups h...

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Detalles Bibliográficos
Autores principales: Malik, Afsheen, Gul, Alvina, Munir, Faiza, Amir, Rabia, Alipour, Hadi, Babar, Mustafeez Mujtaba, Bakhtiar, Syeda Marriam, Paracha, Rehan Zafar, Khalid, Zoya, Hayat, Muhammad Qasim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Bioinformatics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142926/
https://www.ncbi.nlm.nih.gov/pubmed/34055482
http://dx.doi.org/10.7717/peerj.11409

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142926/
https://www.ncbi.nlm.nih.gov/pubmed/34055482
http://dx.doi.org/10.7717/peerj.11409

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