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Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields

Non-uniform illumination limits quantitative analyses of fluorescence imaging techniques. In particular, single molecule localization microscopy (SMLM) relies on high irradiances, but conventional Gaussian-shaped laser illumination restricts the usable field of view to around 40 µm × 40 µm. We prese...

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Detalles Bibliográficos
Autores principales: Mau, Adrien, Friedl, Karoline, Leterrier, Christophe, Bourg, Nicolas, Lévêque-Fort, Sandrine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144377/
https://www.ncbi.nlm.nih.gov/pubmed/34031402
http://dx.doi.org/10.1038/s41467-021-23405-4