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Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields
Non-uniform illumination limits quantitative analyses of fluorescence imaging techniques. In particular, single molecule localization microscopy (SMLM) relies on high irradiances, but conventional Gaussian-shaped laser illumination restricts the usable field of view to around 40 µm × 40 µm. We prese...
Autores principales: | Mau, Adrien, Friedl, Karoline, Leterrier, Christophe, Bourg, Nicolas, Lévêque-Fort, Sandrine |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144377/ https://www.ncbi.nlm.nih.gov/pubmed/34031402 http://dx.doi.org/10.1038/s41467-021-23405-4 |
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