Characterization of a Dye-Decolorizing Peroxidase from Irpex lacteus Expressed in Escherichia coli: An Enzyme with Wide Substrate Specificity Able to Transform Lignosulfonates

A dye-decolorizing peroxidase (DyP) from Irpex lacteus was cloned and heterologously expressed as inclusion bodies in Escherichia coli. The protein was purified in one chromatographic step after its in vitro activation. It was active on ABTS, 2,6-dimethoxyphenol (DMP), and anthraquinoid and azo dyes...

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Detalles Bibliográficos
Autores principales: de Eugenio, Laura Isabel, Peces-Pérez, Rosa, Linde, Dolores, Prieto, Alicia, Barriuso, Jorge, Ruiz-Dueñas, Francisco Javier, Martínez, María Jesús
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145141/
https://www.ncbi.nlm.nih.gov/pubmed/33922393
http://dx.doi.org/10.3390/jof7050325

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145141/
https://www.ncbi.nlm.nih.gov/pubmed/33922393
http://dx.doi.org/10.3390/jof7050325

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