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Characterization of a Dye-Decolorizing Peroxidase from Irpex lacteus Expressed in Escherichia coli: An Enzyme with Wide Substrate Specificity Able to Transform Lignosulfonates
A dye-decolorizing peroxidase (DyP) from Irpex lacteus was cloned and heterologously expressed as inclusion bodies in Escherichia coli. The protein was purified in one chromatographic step after its in vitro activation. It was active on ABTS, 2,6-dimethoxyphenol (DMP), and anthraquinoid and azo dyes...
Autores principales: | de Eugenio, Laura Isabel, Peces-Pérez, Rosa, Linde, Dolores, Prieto, Alicia, Barriuso, Jorge, Ruiz-Dueñas, Francisco Javier, Martínez, María Jesús |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145141/ https://www.ncbi.nlm.nih.gov/pubmed/33922393 http://dx.doi.org/10.3390/jof7050325 |
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