Reversible RNA acylation for control of CRISPR–Cas9 gene editing

We report the development of post-transcriptional chemical methods that enable control over CRISPR–Cas9 gene editing activity both in in vitro assays and in living cells. We show that an azide-substituted acyl imidazole reagent (NAI-N(3)) efficiently acylates CRISPR single guide RNAs (sgRNAs) in 20...

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Detalles Bibliográficos
Autores principales: Habibian, Maryam, McKinlay, Colin, Blake, Timothy R., Kietrys, Anna M., Waymouth, Robert M., Wender, Paul A., Kool, Eric T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145180/
https://www.ncbi.nlm.nih.gov/pubmed/34084356
http://dx.doi.org/10.1039/c9sc03639c

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145180/
https://www.ncbi.nlm.nih.gov/pubmed/34084356
http://dx.doi.org/10.1039/c9sc03639c

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