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Inhibition of K-Ras4B-plasma membrane association with a membrane microdomain-targeting peptide
The association of K-Ras4B protein with plasma membrane (PM) is required for its signaling activity. Thus, direct inhibition of K-Ras4B–PM interaction could be a potential anti-Ras therapeutic strategy. However, it remains challenging to modulate such protein–PM interaction. Based on Ras isoform-spe...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145430/ https://www.ncbi.nlm.nih.gov/pubmed/34123058 http://dx.doi.org/10.1039/c9sc04726c |
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author | Li, Fang-Yi Zhang, Zhen-Feng Voss, Stephanie Wu, Yao-Wen Zhao, Yu-Fen Li, Yan-Mei Chen, Yong-Xiang |
author_facet | Li, Fang-Yi Zhang, Zhen-Feng Voss, Stephanie Wu, Yao-Wen Zhao, Yu-Fen Li, Yan-Mei Chen, Yong-Xiang |
author_sort | Li, Fang-Yi |
collection | PubMed |
description | The association of K-Ras4B protein with plasma membrane (PM) is required for its signaling activity. Thus, direct inhibition of K-Ras4B–PM interaction could be a potential anti-Ras therapeutic strategy. However, it remains challenging to modulate such protein–PM interaction. Based on Ras isoform-specific PM microdomain localization patterns, we have developed a potent and isoform-selective peptide inhibitor, Memrasin, for detachment of K-Ras4B from the PM. Memrasin is one of the first direct inhibitors of K-Ras4B–PM interaction, and consists of a membrane l(d) region-binding sequence derived from the C-terminal region of K-Ras4B and an endosome-escape enhancing motif that can aggregate on membrane. It forms peptide-enriched domains in the l(d) region, abrogates the tethering of K-Ras4B to the PM and accordingly impairs Ras signaling activity, thereby efficiently decreasing the viability of several human lung cancer cells in a dose-responsive and K-Ras dependent manner. Memrasin provides a useful tool for exploring the biological function of K-Ras4B on or off the PM and a potential starting point for further development into anti-Ras therapeutics. |
format | Online Article Text |
id | pubmed-8145430 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-81454302021-06-11 Inhibition of K-Ras4B-plasma membrane association with a membrane microdomain-targeting peptide Li, Fang-Yi Zhang, Zhen-Feng Voss, Stephanie Wu, Yao-Wen Zhao, Yu-Fen Li, Yan-Mei Chen, Yong-Xiang Chem Sci Chemistry The association of K-Ras4B protein with plasma membrane (PM) is required for its signaling activity. Thus, direct inhibition of K-Ras4B–PM interaction could be a potential anti-Ras therapeutic strategy. However, it remains challenging to modulate such protein–PM interaction. Based on Ras isoform-specific PM microdomain localization patterns, we have developed a potent and isoform-selective peptide inhibitor, Memrasin, for detachment of K-Ras4B from the PM. Memrasin is one of the first direct inhibitors of K-Ras4B–PM interaction, and consists of a membrane l(d) region-binding sequence derived from the C-terminal region of K-Ras4B and an endosome-escape enhancing motif that can aggregate on membrane. It forms peptide-enriched domains in the l(d) region, abrogates the tethering of K-Ras4B to the PM and accordingly impairs Ras signaling activity, thereby efficiently decreasing the viability of several human lung cancer cells in a dose-responsive and K-Ras dependent manner. Memrasin provides a useful tool for exploring the biological function of K-Ras4B on or off the PM and a potential starting point for further development into anti-Ras therapeutics. The Royal Society of Chemistry 2019-12-03 /pmc/articles/PMC8145430/ /pubmed/34123058 http://dx.doi.org/10.1039/c9sc04726c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Li, Fang-Yi Zhang, Zhen-Feng Voss, Stephanie Wu, Yao-Wen Zhao, Yu-Fen Li, Yan-Mei Chen, Yong-Xiang Inhibition of K-Ras4B-plasma membrane association with a membrane microdomain-targeting peptide |
title | Inhibition of K-Ras4B-plasma membrane association with a membrane microdomain-targeting peptide |
title_full | Inhibition of K-Ras4B-plasma membrane association with a membrane microdomain-targeting peptide |
title_fullStr | Inhibition of K-Ras4B-plasma membrane association with a membrane microdomain-targeting peptide |
title_full_unstemmed | Inhibition of K-Ras4B-plasma membrane association with a membrane microdomain-targeting peptide |
title_short | Inhibition of K-Ras4B-plasma membrane association with a membrane microdomain-targeting peptide |
title_sort | inhibition of k-ras4b-plasma membrane association with a membrane microdomain-targeting peptide |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145430/ https://www.ncbi.nlm.nih.gov/pubmed/34123058 http://dx.doi.org/10.1039/c9sc04726c |
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