Universal Detection of Mi(a) Antigen and Frequencies of Glycophorin Hybrids among Blood Donors in Taiwan by Human Monoclonal Antibodies against Mi(a) (MNS7), Mur (MNS10), and MUT (MNS35) Antigens

Glycophorin hybrids such as GP.Mur are common in Southeast Asians. In Taiwan, clinically significant alloantibodies to the GP.Mur phenotype are the most important issue in blood banks. A large-scale screening of glycophorin hybrids in the Taiwanese population is urgently needed to ensure transfusion...

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Autores principales: Yang, Meng-Hua, Chen, Jen-Wei, Sayaka, Kaito, Uchikawa, Makoto, Tsuno, Nelson H., Wei, Sheng-Tang, Hou, Sheng-Mou, Chen, Yann-Jang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146793/
https://www.ncbi.nlm.nih.gov/pubmed/33946749
http://dx.doi.org/10.3390/diagnostics11050806
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author Yang, Meng-Hua
Chen, Jen-Wei
Sayaka, Kaito
Uchikawa, Makoto
Tsuno, Nelson H.
Wei, Sheng-Tang
Hou, Sheng-Mou
Chen, Yann-Jang
author_facet Yang, Meng-Hua
Chen, Jen-Wei
Sayaka, Kaito
Uchikawa, Makoto
Tsuno, Nelson H.
Wei, Sheng-Tang
Hou, Sheng-Mou
Chen, Yann-Jang
author_sort Yang, Meng-Hua
collection PubMed
description Glycophorin hybrids such as GP.Mur are common in Southeast Asians. In Taiwan, clinically significant alloantibodies to the GP.Mur phenotype are the most important issue in blood banks. A large-scale screening of glycophorin hybrids in the Taiwanese population is urgently needed to ensure transfusion safety. Four clones of human hybridomas that secrete anti-Mi(a), anti-MUT, and anti-Mur were established by fusing human B-lymphocytes and myeloma cells (JMS-3). The specificity of each monoclonal antibody (MoAb) was characterized. Three MoAbs were applied on an Automated Pretransfusion Blood Testing Analyzer (PK7300/PK7400) for donor screening. Genotyping was performed to determine the detailed subgrouping of glycophorin hybrids. Four MoAbs are IgM antibodies. Anti-Mi(a) (377T) binds to (46)DXHKRDTYA(54), (48)HKRDTYAAHT(57) peptides, and anti-Mi(a) (367T) binds to (43)QTNDXHKRD(51) peptides (X indicates T, M, or K). Anti-Mur is reactive with (49)KRDTYPAHTA(58) peptides. Anti-MUT is reactive with (47)KHKRDTYA(54). A total of 78,327 donors were screened using three MoAbs, and 3690 (4.71%) were GP.Mur, 20 (0.025%) were GP.Hut, and 18 (0.022%) were GP.Vw. When the Mi(a) antigen was introduced as routine screening, the frequency of Mi(a+) among blood donors in Taiwan was 4.66% (67,348/1,444,541). Mi(a) antigen was implemented as a routine blood testing, and the results were labeled on all red blood cell (RBC) units.
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spelling pubmed-81467932021-05-26 Universal Detection of Mi(a) Antigen and Frequencies of Glycophorin Hybrids among Blood Donors in Taiwan by Human Monoclonal Antibodies against Mi(a) (MNS7), Mur (MNS10), and MUT (MNS35) Antigens Yang, Meng-Hua Chen, Jen-Wei Sayaka, Kaito Uchikawa, Makoto Tsuno, Nelson H. Wei, Sheng-Tang Hou, Sheng-Mou Chen, Yann-Jang Diagnostics (Basel) Article Glycophorin hybrids such as GP.Mur are common in Southeast Asians. In Taiwan, clinically significant alloantibodies to the GP.Mur phenotype are the most important issue in blood banks. A large-scale screening of glycophorin hybrids in the Taiwanese population is urgently needed to ensure transfusion safety. Four clones of human hybridomas that secrete anti-Mi(a), anti-MUT, and anti-Mur were established by fusing human B-lymphocytes and myeloma cells (JMS-3). The specificity of each monoclonal antibody (MoAb) was characterized. Three MoAbs were applied on an Automated Pretransfusion Blood Testing Analyzer (PK7300/PK7400) for donor screening. Genotyping was performed to determine the detailed subgrouping of glycophorin hybrids. Four MoAbs are IgM antibodies. Anti-Mi(a) (377T) binds to (46)DXHKRDTYA(54), (48)HKRDTYAAHT(57) peptides, and anti-Mi(a) (367T) binds to (43)QTNDXHKRD(51) peptides (X indicates T, M, or K). Anti-Mur is reactive with (49)KRDTYPAHTA(58) peptides. Anti-MUT is reactive with (47)KHKRDTYA(54). A total of 78,327 donors were screened using three MoAbs, and 3690 (4.71%) were GP.Mur, 20 (0.025%) were GP.Hut, and 18 (0.022%) were GP.Vw. When the Mi(a) antigen was introduced as routine screening, the frequency of Mi(a+) among blood donors in Taiwan was 4.66% (67,348/1,444,541). Mi(a) antigen was implemented as a routine blood testing, and the results were labeled on all red blood cell (RBC) units. MDPI 2021-04-29 /pmc/articles/PMC8146793/ /pubmed/33946749 http://dx.doi.org/10.3390/diagnostics11050806 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Meng-Hua
Chen, Jen-Wei
Sayaka, Kaito
Uchikawa, Makoto
Tsuno, Nelson H.
Wei, Sheng-Tang
Hou, Sheng-Mou
Chen, Yann-Jang
Universal Detection of Mi(a) Antigen and Frequencies of Glycophorin Hybrids among Blood Donors in Taiwan by Human Monoclonal Antibodies against Mi(a) (MNS7), Mur (MNS10), and MUT (MNS35) Antigens
title Universal Detection of Mi(a) Antigen and Frequencies of Glycophorin Hybrids among Blood Donors in Taiwan by Human Monoclonal Antibodies against Mi(a) (MNS7), Mur (MNS10), and MUT (MNS35) Antigens
title_full Universal Detection of Mi(a) Antigen and Frequencies of Glycophorin Hybrids among Blood Donors in Taiwan by Human Monoclonal Antibodies against Mi(a) (MNS7), Mur (MNS10), and MUT (MNS35) Antigens
title_fullStr Universal Detection of Mi(a) Antigen and Frequencies of Glycophorin Hybrids among Blood Donors in Taiwan by Human Monoclonal Antibodies against Mi(a) (MNS7), Mur (MNS10), and MUT (MNS35) Antigens
title_full_unstemmed Universal Detection of Mi(a) Antigen and Frequencies of Glycophorin Hybrids among Blood Donors in Taiwan by Human Monoclonal Antibodies against Mi(a) (MNS7), Mur (MNS10), and MUT (MNS35) Antigens
title_short Universal Detection of Mi(a) Antigen and Frequencies of Glycophorin Hybrids among Blood Donors in Taiwan by Human Monoclonal Antibodies against Mi(a) (MNS7), Mur (MNS10), and MUT (MNS35) Antigens
title_sort universal detection of mi(a) antigen and frequencies of glycophorin hybrids among blood donors in taiwan by human monoclonal antibodies against mi(a) (mns7), mur (mns10), and mut (mns35) antigens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8146793/
https://www.ncbi.nlm.nih.gov/pubmed/33946749
http://dx.doi.org/10.3390/diagnostics11050806
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