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Analysis and Optimization of Conditions for the Use of 2′,7′-Dichlorofluorescein Diacetate in Cultured Hepatocytes

Numerous liver pathologies encompass oxidative stress as molecular basis of disease. The use of 2′,7′-dichlorodihydrofluorescein-diacetate (DCFH(2)-DA) as fluorogenic redox probe is problematic in liver cell lines because of membrane transport proteins that interfere with probe kinetics, among other...

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Detalles Bibliográficos
Autores principales: Reiniers, Megan J., de Haan, Lianne R., Reeskamp, Laurens F., Broekgaarden, Mans, van Golen, Rowan F., Heger, Michal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147027/
https://www.ncbi.nlm.nih.gov/pubmed/33925917
http://dx.doi.org/10.3390/antiox10050674