Cargando…
Analysis and Optimization of Conditions for the Use of 2′,7′-Dichlorofluorescein Diacetate in Cultured Hepatocytes
Numerous liver pathologies encompass oxidative stress as molecular basis of disease. The use of 2′,7′-dichlorodihydrofluorescein-diacetate (DCFH(2)-DA) as fluorogenic redox probe is problematic in liver cell lines because of membrane transport proteins that interfere with probe kinetics, among other...
Autores principales: | Reiniers, Megan J., de Haan, Lianne R., Reeskamp, Laurens F., Broekgaarden, Mans, van Golen, Rowan F., Heger, Michal |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147027/ https://www.ncbi.nlm.nih.gov/pubmed/33925917 http://dx.doi.org/10.3390/antiox10050674 |
Ejemplares similares
-
Experimental Conditions That Influence the Utility of 2′7′-Dichlorodihydrofluorescein Diacetate (DCFH(2)-DA) as a Fluorogenic Biosensor for Mitochondrial Redox Status
por: de Haan, Lianne R., et al.
Publicado: (2022) -
Preparation and Practical Applications of 2′,7′-Dichlorodihydrofluorescein
in Redox Assays
por: Reiniers, Megan J., et al.
Publicado: (2017) -
A 2,7-dichlorofluorescein derivative to monitor microcalcifications
por: Tholen, Patrik, et al.
Publicado: (2022) -
Determination of urea in milk based on N-bromosuccinimide–dichlorofluorescein postchemiluminescence method
por: Nie, Fei, et al.
Publicado: (2016) -
Metal-Sulfate Induced Generation of ROS in Human Brain Cells: Detection Using an Isomeric Mixture of 5- and 6-Carboxy-2′,7′-Dichlorofluorescein Diacetate (Carboxy-DCFDA) as a Cell Permeant Tracer
por: Pogue, Aileen I., et al.
Publicado: (2012)